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purelink quick gel extraction kit k210012  (Thermo Fisher)


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    Thermo Fisher purelink quick gel extraction kit k210012
    Purelink Quick Gel Extraction Kit K210012, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purelink+quick+gel+extraction+kit/purelink+rna+mini+kit/pm40412790-71-6-12
    Average 90 stars, based on 1 article reviews
    purelink quick gel extraction kit k210012 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Novel Method for the Rapid Establishment of Antibiotic Susceptibility Profiles in Bacterial Strains Linked to Musculoskeletal Infections Using Scattered Light Integrated Collector Technology.
    Article Snippet: The ribosomal 16S genomic sequence was amplified using conventional PCR followed by electrophoresis on a 1.5% agarose gel containing PeqGreen RNA/DNA dye (PeqLab Biotechnologie GmbH, Erlangen, Germany) and imaged using a ChemiDoc MP scanner (BioRad, Feldkirchen, Germany). .. PCR products were purified from the gel using a Purelink Quick Gel Extraction kit (Thermo Fisher Scientific, Darmstadt, Germany) following the manufacturer’s instructions. .. Finally, samples were sent for Sanger sequencing (Eurofins Genomics, Ebersberg, Germany).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy
    Article Snippet: .. All PCR products were purified using the PureLink PCR Purification Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), digested with Sfi I (New England Biolabs, Whitby, ON, Canada), and gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). .. Equal concentrations of the purified flanking regions and resistance cassette were ligated using T4 DNA ligase (New England Biolabs, Whitby ON, Canada) and were purified using the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy
    Article Snippet: .. The PCR product and p123 were digested with Kpn I and Xma I (New England Biolabs, Whitby, ON, Canada) and purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada) following the manufacturer’s protocols. .. The digested and purified PCR product and p123 without P otef were ligated with T4 DNA ligase, transformed into Subcloning Efficiency DH5α Competent Escherichia coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada), and plated on LB Broth (Miller) (MilliporeSigma, Oakville, ON, Canada) supplemented with 100 μg/mL ampicillin following the manufacturer’s protocols.

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: Confirmed deletion constructs were PCR amplified from pCR2.1TOPO∆UMAG_01732HygR and pCR2.1TOPO∆UMAG_01732-CarbR using primers Dbp3_LF_Nested-F and Dbp3_RF-R (Table S2) and Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Mississauga, ON, Canada). .. PCR products were gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). ..

    Article Title: A new sequential dual flow lab-on-a-chip with a lyophilized one-component chemiluminescence substrate for high-sensitive microchannel lateral flow assay (mLFA)
    Article Snippet: The first polymerase chain reaction (PCR) was performed to amplify the VL and VH chain genes using cDNA and degenerative primer sets, as described previously by Lin et al.27 The PCR products were analyzed by agarose gel electrophoresis, and the approximately 400 bp and 350 bp bands corresponding to VH and VL genes, respectively, were excised from the gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. A second PCR was performed to join the VH and VL genes with a flexible (G4S)3 linker (scFv), as described previously by Lin et al.27 The resulting approximately 750 bp PCR products were excised from the agarose gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. The purified PCR products were digested with NotI and BglI restriction enzymes (Fermentas) and ligated into 50 ng of the digested pADL100 phagemid (Antibody Design Laboratories, USA) using T4 DNA ligase (ThermoFisher, USA).

    Purification:

    Article Title: Novel Method for the Rapid Establishment of Antibiotic Susceptibility Profiles in Bacterial Strains Linked to Musculoskeletal Infections Using Scattered Light Integrated Collector Technology.
    Article Snippet: The ribosomal 16S genomic sequence was amplified using conventional PCR followed by electrophoresis on a 1.5% agarose gel containing PeqGreen RNA/DNA dye (PeqLab Biotechnologie GmbH, Erlangen, Germany) and imaged using a ChemiDoc MP scanner (BioRad, Feldkirchen, Germany). .. PCR products were purified from the gel using a Purelink Quick Gel Extraction kit (Thermo Fisher Scientific, Darmstadt, Germany) following the manufacturer’s instructions. .. Finally, samples were sent for Sanger sequencing (Eurofins Genomics, Ebersberg, Germany).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: 2025, 26, 2432 20 of 28 the PureLink PCR Purification Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), digested with SfiI (New England Biolabs, Whitby, ON, Canada), and gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). .. Equal concentrations of the purified flanking regions and resistance cassette were ligated using T4 DNA ligase (New England Biolabs, Whitby ON, Canada) and were purified using the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). .. The HygR and CarbR constructs were cloned into the pCR2.1 TOPO vector and transformed into One Shot TOP10 competent E. coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: Both p123 + crg1 and PCR products were digested with NcoI-HF and NotI-HF (New England Biolabs, Whitby, ON, Canada). .. Digested products were gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), followed by ligation with T4 DNA ligase (New England Biolabs, Whitby, ON, Canada). .. The ligated product produced the p123 + crg1 + uded1 construct which was transformed into Subcloning Efficiency DH5α Competent Escherichia coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada) and grown on LB Broth (Miller) (MilliporeSigma, Oakville, ON, Canada) plates supplemented with 100 μg/mL ampicillin with modifications to the manufacturer’s protocols.

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy
    Article Snippet: .. All PCR products were purified using the PureLink PCR Purification Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), digested with Sfi I (New England Biolabs, Whitby, ON, Canada), and gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). .. Equal concentrations of the purified flanking regions and resistance cassette were ligated using T4 DNA ligase (New England Biolabs, Whitby ON, Canada) and were purified using the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy
    Article Snippet: .. The PCR product and p123 were digested with Kpn I and Xma I (New England Biolabs, Whitby, ON, Canada) and purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada) following the manufacturer’s protocols. .. The digested and purified PCR product and p123 without P otef were ligated with T4 DNA ligase, transformed into Subcloning Efficiency DH5α Competent Escherichia coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada), and plated on LB Broth (Miller) (MilliporeSigma, Oakville, ON, Canada) supplemented with 100 μg/mL ampicillin following the manufacturer’s protocols.

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: Confirmed deletion constructs were PCR amplified from pCR2.1TOPO∆UMAG_01732HygR and pCR2.1TOPO∆UMAG_01732-CarbR using primers Dbp3_LF_Nested-F and Dbp3_RF-R (Table S2) and Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Mississauga, ON, Canada). .. PCR products were gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). ..

    Article Title: A new sequential dual flow lab-on-a-chip with a lyophilized one-component chemiluminescence substrate for high-sensitive microchannel lateral flow assay (mLFA)
    Article Snippet: The first polymerase chain reaction (PCR) was performed to amplify the VL and VH chain genes using cDNA and degenerative primer sets, as described previously by Lin et al.27 The PCR products were analyzed by agarose gel electrophoresis, and the approximately 400 bp and 350 bp bands corresponding to VH and VL genes, respectively, were excised from the gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. A second PCR was performed to join the VH and VL genes with a flexible (G4S)3 linker (scFv), as described previously by Lin et al.27 The resulting approximately 750 bp PCR products were excised from the agarose gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. The purified PCR products were digested with NotI and BglI restriction enzymes (Fermentas) and ligated into 50 ng of the digested pADL100 phagemid (Antibody Design Laboratories, USA) using T4 DNA ligase (ThermoFisher, USA).

    Gel Extraction:

    Article Title: Novel Method for the Rapid Establishment of Antibiotic Susceptibility Profiles in Bacterial Strains Linked to Musculoskeletal Infections Using Scattered Light Integrated Collector Technology.
    Article Snippet: The ribosomal 16S genomic sequence was amplified using conventional PCR followed by electrophoresis on a 1.5% agarose gel containing PeqGreen RNA/DNA dye (PeqLab Biotechnologie GmbH, Erlangen, Germany) and imaged using a ChemiDoc MP scanner (BioRad, Feldkirchen, Germany). .. PCR products were purified from the gel using a Purelink Quick Gel Extraction kit (Thermo Fisher Scientific, Darmstadt, Germany) following the manufacturer’s instructions. .. Finally, samples were sent for Sanger sequencing (Eurofins Genomics, Ebersberg, Germany).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: 2025, 26, 2432 20 of 28 the PureLink PCR Purification Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), digested with SfiI (New England Biolabs, Whitby, ON, Canada), and gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). .. Equal concentrations of the purified flanking regions and resistance cassette were ligated using T4 DNA ligase (New England Biolabs, Whitby ON, Canada) and were purified using the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). .. The HygR and CarbR constructs were cloned into the pCR2.1 TOPO vector and transformed into One Shot TOP10 competent E. coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: Both p123 + crg1 and PCR products were digested with NcoI-HF and NotI-HF (New England Biolabs, Whitby, ON, Canada). .. Digested products were gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), followed by ligation with T4 DNA ligase (New England Biolabs, Whitby, ON, Canada). .. The ligated product produced the p123 + crg1 + uded1 construct which was transformed into Subcloning Efficiency DH5α Competent Escherichia coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada) and grown on LB Broth (Miller) (MilliporeSigma, Oakville, ON, Canada) plates supplemented with 100 μg/mL ampicillin with modifications to the manufacturer’s protocols.

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy
    Article Snippet: .. All PCR products were purified using the PureLink PCR Purification Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), digested with Sfi I (New England Biolabs, Whitby, ON, Canada), and gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). .. Equal concentrations of the purified flanking regions and resistance cassette were ligated using T4 DNA ligase (New England Biolabs, Whitby ON, Canada) and were purified using the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada).

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy
    Article Snippet: .. The PCR product and p123 were digested with Kpn I and Xma I (New England Biolabs, Whitby, ON, Canada) and purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada) following the manufacturer’s protocols. .. The digested and purified PCR product and p123 without P otef were ligated with T4 DNA ligase, transformed into Subcloning Efficiency DH5α Competent Escherichia coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada), and plated on LB Broth (Miller) (MilliporeSigma, Oakville, ON, Canada) supplemented with 100 μg/mL ampicillin following the manufacturer’s protocols.

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: Confirmed deletion constructs were PCR amplified from pCR2.1TOPO∆UMAG_01732HygR and pCR2.1TOPO∆UMAG_01732-CarbR using primers Dbp3_LF_Nested-F and Dbp3_RF-R (Table S2) and Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Mississauga, ON, Canada). .. PCR products were gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada). ..

    Article Title: A new sequential dual flow lab-on-a-chip with a lyophilized one-component chemiluminescence substrate for high-sensitive microchannel lateral flow assay (mLFA)
    Article Snippet: The first polymerase chain reaction (PCR) was performed to amplify the VL and VH chain genes using cDNA and degenerative primer sets, as described previously by Lin et al.27 The PCR products were analyzed by agarose gel electrophoresis, and the approximately 400 bp and 350 bp bands corresponding to VH and VL genes, respectively, were excised from the gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. A second PCR was performed to join the VH and VL genes with a flexible (G4S)3 linker (scFv), as described previously by Lin et al.27 The resulting approximately 750 bp PCR products were excised from the agarose gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. The purified PCR products were digested with NotI and BglI restriction enzymes (Fermentas) and ligated into 50 ng of the digested pADL100 phagemid (Antibody Design Laboratories, USA) using T4 DNA ligase (ThermoFisher, USA).

    Ligation:

    Article Title: Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
    Article Snippet: Both p123 + crg1 and PCR products were digested with NcoI-HF and NotI-HF (New England Biolabs, Whitby, ON, Canada). .. Digested products were gel purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, Mississauga, ON, Canada), followed by ligation with T4 DNA ligase (New England Biolabs, Whitby, ON, Canada). .. The ligated product produced the p123 + crg1 + uded1 construct which was transformed into Subcloning Efficiency DH5α Competent Escherichia coli cells (Thermo Fisher Scientific, Mississauga, ON, Canada) and grown on LB Broth (Miller) (MilliporeSigma, Oakville, ON, Canada) plates supplemented with 100 μg/mL ampicillin with modifications to the manufacturer’s protocols.

    other:

    Article Title: Synergistic removal of emerging contaminants using bacterial augmented floating treatment bed system (FTBs) of Typha latifolia and Canna indica for rejuvenation of polluted river water
    Article Snippet: The InvitrogenTM PureLinkTM Quick Gel Extraction Kit (Thermo Fisher Scientific, USA) was used to extract the PCR products for cloning.

    Agarose Gel Electrophoresis:

    Article Title: A new sequential dual flow lab-on-a-chip with a lyophilized one-component chemiluminescence substrate for high-sensitive microchannel lateral flow assay (mLFA)
    Article Snippet: The first polymerase chain reaction (PCR) was performed to amplify the VL and VH chain genes using cDNA and degenerative primer sets, as described previously by Lin et al.27 The PCR products were analyzed by agarose gel electrophoresis, and the approximately 400 bp and 350 bp bands corresponding to VH and VL genes, respectively, were excised from the gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. A second PCR was performed to join the VH and VL genes with a flexible (G4S)3 linker (scFv), as described previously by Lin et al.27 The resulting approximately 750 bp PCR products were excised from the agarose gel and purified using a PureLink Quick Gel Extraction Kit (ThermoFisher, USA). .. The purified PCR products were digested with NotI and BglI restriction enzymes (Fermentas) and ligated into 50 ng of the digested pADL100 phagemid (Antibody Design Laboratories, USA) using T4 DNA ligase (ThermoFisher, USA).



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